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| Effects of feeding practices on distribution characteristics of intestinal microbiota in preterm newborns |
| YUAN Fang1, HU Runfang1, WU Liangxia2, ZHANG Jianhua3 |
| 1. Department of Pediatrics, United Family Healthcare, Shanghai 200335, China; 2. Department of Pediatrics, Shanghai Sixth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200233, China; 3. Department of Pediatric Respiratory Medicine, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China |
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Abstract Objective To study the effects of different feeding practices on intestinal microbiota distribution characteristics in preterm infants. Methods A total of 35 preterm infants delivered by cesarean section were recruited into 2 feeding groups fed by breast milk or formula. The total DNA was extracted from the feces, the amplification of the variable regions 3 of the 16S rRNA gene was sequenced through Illumina Miseq sequencing platform and then the profile of the intestinal flora composition was analyzed. Results The intestinal microbiota of premature infants was dominated by two phyla （Firmicutes and Proteobacteria） and three classes （Bacilli, Clostridia, and Gammaproteobacteria）. In the family and genus levels, it was dominated by Streptococcaceae, Veillonellaceae, and Enterobacteriaceae. We did not find Bacteroidetes and Bifidobacterium in the whole observation period. There was no significant difference in relative abundance of the main phylum and genus between the two feeding types. In the low abundance species level, the breast feeding group owned more species than the formula feeding group. The relative abundance of Lactobacillus of the breast feeding group was significantly higher than that of the formula feeding group （P<0.05）. Escherichia was not found in the formula feeding group. In addition, the composition of bacteria in these two groups at four time points was also different and the difference in the phylum level was found in 3 to 5 days after birth. Conclusion High-throughput sequencing reveals the intestinal flora profile of preterm infants: more facultative anaerobe colonization and delayed strict anaerobic bacteria colonization. Feeding type has little effect on the structure of main species. Formula feeding has adverse effects on the establishment of low abundance microbiota.
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