Objective To explore the characteristics of fine motor ability in premature infants and provide the basis for the fine motor training. Methods The 86 premature infants from Obstetrics and Gynecology Hospital affiliated to Nanjing Medical University from April to November, 2016 were assessed by the Hong Kong preschool fine motor developmental assessment. Results There were no one good (0.0%); 36 premature infants were common (37.2%); 10 premature infants were poor (9.3%); and 40 premature infants were extremely poor (53.5%). Conclusion The fine motor ability of premature infants far lags behind  that of peers. It is helpful to early detection, early intervention and to improve the manipulative skills by using fine motor assessment.