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| Establishment of lead-exposed rat models at different developmental stages |
| LI Shufang1, SUN Shuangyuan2, JI Xiaofan2, XU Jian1* |
| 1.MOE-Shanghai Key Laboratory of Children's Environmental Health, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China; 2. Shanghai Municipal Center for Disease Control & Prevention, Shanghai 200336, China |
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Abstract Objective To establish the rat models of developmental lead exposure at different age groups and offer a footstone for the follow-up studies. Methods Two kinds of animal models of developmental lead exposure were established: the model lead-exposed during childhood (20 to 40 days old) and the model leadexposed during adolescence (40 to 60 days old). Eighteen Sprague-Dawley rats were randomly divided into control, low lead-exposed (treated by 0.05% lead acetate in their drinking water for 20 days) and high lead-exposed (treated by 0.2% lead acetate in their drinking water for 20 days) group in both models, 6 in each group. The blood lead levels and bone lead levels (lead levels in cancellous bone and compact bone were respectively measured) were measured to evaluate lead poisoning levels in rats. Results The blood lead levels and bone lead levels of lead exposure groups in both childhood and adolescence models were significantly higher than those of control groups (P<0.01). What's more, in both models, the blood lead levels of high lead-exposed groups were significantly higher than those of low lead-exposed groups (P<0.01). In lead exposure groups, the blood lead levels and lead levels in compact bone of rats in childhood model were higher than those in adolescence model (P<0.01). But, lead levels in cancellous bone had no significant differences (P=0.245). In each group, the blood lead levels and bone lead levels were highly correlated (compact bone: r=0.931, P=0.000；cancellous bone: r=0.918, P=0.000). Conclusion Different levels of lead exposure are needed to establish rat models with same blood or bone lead levels among different age groups.
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